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antibodies against col2a1  (Proteintech)


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    Structured Review

    Proteintech antibodies against col2a1
    Antibodies Against Col2a1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 601 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+collagen+1/Collagen+Type+II+Antibody/pm42809989-127-9-14
    Average 96 stars, based on 601 article reviews
    antibodies against col2a1 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Immunohistochemical staining:

    Article Title: Ergosterol from edible fungi: Enhancing fatty acid oxidation via CPT1A to protect against diabetic kidney disease.
    Article Snippet: Background: Diabetic kidney disease (DKD), a common microvascular complication of diabetes mellitus, is recognized as a leading cause of end-stage renal disease.. Ergosterol, a natural sterol abundant in edible fungi, has shown pharmacological effects that may benefit DKD treatment.. However, its precise mechanisms of action remain elusive.

    Staining:

    Article Title: Ergosterol from edible fungi: Enhancing fatty acid oxidation via CPT1A to protect against diabetic kidney disease.
    Article Snippet: Background: Diabetic kidney disease (DKD), a common microvascular complication of diabetes mellitus, is recognized as a leading cause of end-stage renal disease.. Ergosterol, a natural sterol abundant in edible fungi, has shown pharmacological effects that may benefit DKD treatment.. However, its precise mechanisms of action remain elusive.

    Article Title: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling
    Article Snippet: .. For IHC and IF staining, paraffin sections of left lung tissue samples were incubated overnight at 4 °C with primary antibodies: anti-SNX3 (Servicebio, China, diluted 1:200, #K009182P), anti-α-SMA (Proteintech, China, diluted 1:200, #23081-1-AP), anti-SP-C (Proteintech, China, diluted 1:200, #10774-1-AP), anti-CD31 (Servicebio, China, diluted 1:200, #GB15063), anti-CD68 (Servicebio, China, diluted 1:200, #GB11309), anti-collagen 1 (Proteintech, China, diluted 1:200, #66761-1-Ig), anti-Wls (Proteintech, China, diluted 1:200, #23081-1-AP), anti-CK-1α (Santa Cruz, USA, diluted 1:200, #SC-74582), and anti-β-catenin (Proteintech, China, diluted 1:200, #51667-2-AP). .. After incubation, the samples were exposed to anti-CY3-tyramide (Servicebio, China, diluted 1:500, #G1223), anti-FITC-tyramide (Servicebio, China, diluted 1:500, #G1222), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Servicebio, China, #G1012).

    Incubation:

    Article Title: Ergosterol from edible fungi: Enhancing fatty acid oxidation via CPT1A to protect against diabetic kidney disease.
    Article Snippet: Background: Diabetic kidney disease (DKD), a common microvascular complication of diabetes mellitus, is recognized as a leading cause of end-stage renal disease.. Ergosterol, a natural sterol abundant in edible fungi, has shown pharmacological effects that may benefit DKD treatment.. However, its precise mechanisms of action remain elusive.

    Article Title: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling
    Article Snippet: .. For IHC and IF staining, paraffin sections of left lung tissue samples were incubated overnight at 4 °C with primary antibodies: anti-SNX3 (Servicebio, China, diluted 1:200, #K009182P), anti-α-SMA (Proteintech, China, diluted 1:200, #23081-1-AP), anti-SP-C (Proteintech, China, diluted 1:200, #10774-1-AP), anti-CD31 (Servicebio, China, diluted 1:200, #GB15063), anti-CD68 (Servicebio, China, diluted 1:200, #GB11309), anti-collagen 1 (Proteintech, China, diluted 1:200, #66761-1-Ig), anti-Wls (Proteintech, China, diluted 1:200, #23081-1-AP), anti-CK-1α (Santa Cruz, USA, diluted 1:200, #SC-74582), and anti-β-catenin (Proteintech, China, diluted 1:200, #51667-2-AP). .. After incubation, the samples were exposed to anti-CY3-tyramide (Servicebio, China, diluted 1:500, #G1223), anti-FITC-tyramide (Servicebio, China, diluted 1:500, #G1222), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Servicebio, China, #G1012).

    Article Title: Dexamethasone: a cheap and convenient way to alleviate subsequent stenosis after thermal injury on ureter
    Article Snippet: .. The sections were successively deparaffinized and hydrated, followed by antigen retrieval at 95 °C for 15 min and immersion in 10% hydrogen peroxide for 10 min. Next, the sections were blocked with 10% normal goat serum at room temperature for 30 min and incubated with primary antibodies using anti-IL-1β (1:500, AF5103; Affinity), anti-α-SMA (1:1,000; ab124964; Abcam), anti-collagen 1 (1:300; 14695-1-AP; Proteintech), anti-fibronectin (1:200; 66042-1-Ig; Proteintech) overnight at 4 °C. .. The sections underwent three rounds of washing with PBS for 5 minutes each, followed by a 50-minute incubation at room temperature with the biotinylated secondary antibody (OriGene, USA).

    other:

    Article Title: Plasma apolipoprotein E protein attenuates pulmonary fibrosis through LRP1 and PLAU dual receptor-mediated TGF-β/Smad inhibition.
    Article Snippet: Cell and lung tissue lysates were prepared using RIPA lysis buffer (Beyotime, Shanghai, China).

    Immunohistochemistry:

    Article Title: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling
    Article Snippet: .. For IHC and IF staining, paraffin sections of left lung tissue samples were incubated overnight at 4 °C with primary antibodies: anti-SNX3 (Servicebio, China, diluted 1:200, #K009182P), anti-α-SMA (Proteintech, China, diluted 1:200, #23081-1-AP), anti-SP-C (Proteintech, China, diluted 1:200, #10774-1-AP), anti-CD31 (Servicebio, China, diluted 1:200, #GB15063), anti-CD68 (Servicebio, China, diluted 1:200, #GB11309), anti-collagen 1 (Proteintech, China, diluted 1:200, #66761-1-Ig), anti-Wls (Proteintech, China, diluted 1:200, #23081-1-AP), anti-CK-1α (Santa Cruz, USA, diluted 1:200, #SC-74582), and anti-β-catenin (Proteintech, China, diluted 1:200, #51667-2-AP). .. After incubation, the samples were exposed to anti-CY3-tyramide (Servicebio, China, diluted 1:500, #G1223), anti-FITC-tyramide (Servicebio, China, diluted 1:500, #G1222), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Servicebio, China, #G1012).

    Fluorescence:

    Article Title: Hyperuricemia suppresses lumican, exacerbating adverse remodeling after myocardial infarction by promoting fibroblast phenotype transition.
    Article Snippet: .. The following antibodies were used: anti-Lumican (A11593, ABclonal, 1:200, Wuhan, China), anti-α-SMA (A5228, Sigma‒Aldrich, 1:200, St. Louis, MO), anti-collagen 1 (14695-1-AP, Proteintech, Wuhan, China), antifibronectin (A12932, ABclonal, 1:200), anti-CD68 (ab955, Abcam), anti-Ly6G (127602, Biolegend, San Diego, CA), anti-CD31 (561810, BD Biosciences, San Jose, CA), antic-TnT (A11826, Invitrogen, Carlsbad, CA), 488 nm Alexa fluorescence-conjugated secondary antibodies (A-11001, Thermo Fisher Scientific, Waltham, MA), and 555 nm Alexa fluorescence-conjugated secondary antibodies (A-21428, Thermo Fisher Scientific). .. According to the manufacturer’s instructions, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using an In Situ Apoptosis Detection Kit (11684817910, Roche, Basel, Switzerland).

    Article Title: Hyperuricemia suppresses lumican, exacerbating adverse remodeling after myocardial infarction by promoting fibroblast phenotype transition
    Article Snippet: .. The following antibodies were used: anti-Lumican (A11593, ABclonal, 1:200, Wuhan, China), anti-α-SMA (A5228, Sigma‒Aldrich, 1:200, St. Louis, MO), anti-collagen 1 (14695-1-AP, Proteintech, Wuhan, China), anti-fibronectin (A12932, ABclonal, 1:200), anti-CD68 (ab955, Abcam), anti-Ly6G (127602, Biolegend, San Diego, CA), anti-CD31 (561810, BD Biosciences, San Jose, CA), anti-c-TnT (A11826, Invitrogen, Carlsbad, CA), 488 nm Alexa fluorescence-conjugated secondary antibodies (A-11001, Thermo Fisher Scientific, Waltham, MA), and 555 nm Alexa fluorescence-conjugated secondary antibodies (A-21428, Thermo Fisher Scientific). .. According to the manufacturer’s instructions, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using an In Situ Apoptosis Detection Kit (11684817910, Roche, Basel, Switzerland).



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    D-EVs Alleviate Cellular Senescence and Restore ECM anabolic/catabolic metabolism in Senescent NPCs. (A) The CCK8 assay was used to determine D-EVs concentrations on cell viability. (B) Flow cytometry analysis of proliferative capacity in the above group, and (C) quantitative analysis. (D) Representative ROS images of senescent NPCs treated with N-EVs, D-EVs, or D-EVs + GW4869. (E) Representative SA-β-Gal images of senescent NPCs treated with N-EVs, D-EVs, or D-EVs + GW4869, and (F) quantitative analysis. (G) Confocal analysis of γ-H2A with IF staining depicting DNA damage in the control, TBHP, N-Evs, or D-EVs group. (H) WB analysis of ECM metabolism–related and aging-related proteins in NPCs following treatment with Control, TBHP, N-Evs, or D-EVs. (I) Western blot analysis of p53, p21, and p16 in senescent NPCs treated with D-EVs, D-CM, or D-CM EV-dep . (J) Confocal analysis of <t>COL2</t> with IF staining in the control, TBHP, N-EVs, or D-EVs group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    Regulation of extracellular matrix metabolism by AdHy@Pae in LPS-induced chondrocytes. (A) Representative immunofluorescence images showing the expression of <t>COL2A1,</t> SOX9, MMP9, and ADAMTS5 in different treatment groups and corresponding (B) quantitative analysis. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
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    Image Search Results


    D-EVs Alleviate Cellular Senescence and Restore ECM anabolic/catabolic metabolism in Senescent NPCs. (A) The CCK8 assay was used to determine D-EVs concentrations on cell viability. (B) Flow cytometry analysis of proliferative capacity in the above group, and (C) quantitative analysis. (D) Representative ROS images of senescent NPCs treated with N-EVs, D-EVs, or D-EVs + GW4869. (E) Representative SA-β-Gal images of senescent NPCs treated with N-EVs, D-EVs, or D-EVs + GW4869, and (F) quantitative analysis. (G) Confocal analysis of γ-H2A with IF staining depicting DNA damage in the control, TBHP, N-Evs, or D-EVs group. (H) WB analysis of ECM metabolism–related and aging-related proteins in NPCs following treatment with Control, TBHP, N-Evs, or D-EVs. (I) Western blot analysis of p53, p21, and p16 in senescent NPCs treated with D-EVs, D-CM, or D-CM EV-dep . (J) Confocal analysis of COL2 with IF staining in the control, TBHP, N-EVs, or D-EVs group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration

    doi: 10.1016/j.bioactmat.2026.02.030

    Figure Lengend Snippet: D-EVs Alleviate Cellular Senescence and Restore ECM anabolic/catabolic metabolism in Senescent NPCs. (A) The CCK8 assay was used to determine D-EVs concentrations on cell viability. (B) Flow cytometry analysis of proliferative capacity in the above group, and (C) quantitative analysis. (D) Representative ROS images of senescent NPCs treated with N-EVs, D-EVs, or D-EVs + GW4869. (E) Representative SA-β-Gal images of senescent NPCs treated with N-EVs, D-EVs, or D-EVs + GW4869, and (F) quantitative analysis. (G) Confocal analysis of γ-H2A with IF staining depicting DNA damage in the control, TBHP, N-Evs, or D-EVs group. (H) WB analysis of ECM metabolism–related and aging-related proteins in NPCs following treatment with Control, TBHP, N-Evs, or D-EVs. (I) Western blot analysis of p53, p21, and p16 in senescent NPCs treated with D-EVs, D-CM, or D-CM EV-dep . (J) Confocal analysis of COL2 with IF staining in the control, TBHP, N-EVs, or D-EVs group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: For immunohistochemistry, the tissue sections were incubated overnight at 4 °C with primary antibodies against COL2 (1:1000, 28459-1-AP, Proteintech) and GPX4 (1:500, 381958, Zen-bio).

    Techniques: CCK-8 Assay, Flow Cytometry, Staining, Control, Western Blot

    Histological evaluation of the D-EVs@Gel ROS in a rat IDD model. (A) Representative histological staining of intervertebral disc tissues (H&E, Safranin O, and Masson) at 4 and 8 weeks post-treatment. Scale bars: 1 mm. (B, E) Immunohistochemical staining and quantification for GPX4, a key inhibitor of ferroptosis, at 4 and 8 weeks post-treatment. (C, F) Immunohistochemical staining and quantification for COL2 at 4 and 8 weeks post-treatment. (D) Quantitative analyses of histological score at 4 and 8 weeks post-treatment. Scale bars: 500 μm. The data were presented as mean ± SD. n = 6, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration

    doi: 10.1016/j.bioactmat.2026.02.030

    Figure Lengend Snippet: Histological evaluation of the D-EVs@Gel ROS in a rat IDD model. (A) Representative histological staining of intervertebral disc tissues (H&E, Safranin O, and Masson) at 4 and 8 weeks post-treatment. Scale bars: 1 mm. (B, E) Immunohistochemical staining and quantification for GPX4, a key inhibitor of ferroptosis, at 4 and 8 weeks post-treatment. (C, F) Immunohistochemical staining and quantification for COL2 at 4 and 8 weeks post-treatment. (D) Quantitative analyses of histological score at 4 and 8 weeks post-treatment. Scale bars: 500 μm. The data were presented as mean ± SD. n = 6, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: For immunohistochemistry, the tissue sections were incubated overnight at 4 °C with primary antibodies against COL2 (1:1000, 28459-1-AP, Proteintech) and GPX4 (1:500, 381958, Zen-bio).

    Techniques: Staining, Immunohistochemical staining

    Regulation of extracellular matrix metabolism by AdHy@Pae in LPS-induced chondrocytes. (A) Representative immunofluorescence images showing the expression of COL2A1, SOX9, MMP9, and ADAMTS5 in different treatment groups and corresponding (B) quantitative analysis. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Journal: Bioactive Materials

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    doi: 10.1016/j.bioactmat.2026.02.051

    Figure Lengend Snippet: Regulation of extracellular matrix metabolism by AdHy@Pae in LPS-induced chondrocytes. (A) Representative immunofluorescence images showing the expression of COL2A1, SOX9, MMP9, and ADAMTS5 in different treatment groups and corresponding (B) quantitative analysis. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100).

    Techniques: Immunofluorescence, Expressing

    Expression analysis of cartilage-related proteins after treatment with AdHy@Pae. (A) Representative Western blot images showing ECM synthesis–related proteins (COL2A1, ACAN, COMP, and SOX9) and antioxidant markers (NRF2 and HO-1) in different groups. (B) Quantitative analysis of matrix-degrading enzymes (MMP13, ADAMTS5, ADAMTS1) and apoptosis-related proteins (BCL-2, Bax, and Caspase-3). Data are presented as mean ± SD (n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Journal: Bioactive Materials

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    doi: 10.1016/j.bioactmat.2026.02.051

    Figure Lengend Snippet: Expression analysis of cartilage-related proteins after treatment with AdHy@Pae. (A) Representative Western blot images showing ECM synthesis–related proteins (COL2A1, ACAN, COMP, and SOX9) and antioxidant markers (NRF2 and HO-1) in different groups. (B) Quantitative analysis of matrix-degrading enzymes (MMP13, ADAMTS5, ADAMTS1) and apoptosis-related proteins (BCL-2, Bax, and Caspase-3). Data are presented as mean ± SD (n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

    Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100).

    Techniques: Expressing, Western Blot

    In vivo validation of the chondroprotective and mitochondrial regulatory effects of AdHy@Pae after therapy for 4 weeks. (A) Representative immunohistochemical staining of cartilage sections for COL2A1, SOX9, MMP3, and ADAMTS1 in different groups (Sham, PBS, Hy, AdHy, and AdHy@Pae). (B) Quantitative analysis of the immunohistochemical staining intensity showing relative expression levels of anabolic (COL2A1, SOX9) and catabolic (MMP3, ADAMTS1) markers. (C) TEM images of chondrocytes showing mitochondrial ultrastructure in each group, with high-magnification views (bottom panels) indicating cristae integrity and membrane morphology. Data are presented as mean ± SD (n = 3); ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗P < 0.0001.

    Journal: Bioactive Materials

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

    doi: 10.1016/j.bioactmat.2026.02.051

    Figure Lengend Snippet: In vivo validation of the chondroprotective and mitochondrial regulatory effects of AdHy@Pae after therapy for 4 weeks. (A) Representative immunohistochemical staining of cartilage sections for COL2A1, SOX9, MMP3, and ADAMTS1 in different groups (Sham, PBS, Hy, AdHy, and AdHy@Pae). (B) Quantitative analysis of the immunohistochemical staining intensity showing relative expression levels of anabolic (COL2A1, SOX9) and catabolic (MMP3, ADAMTS1) markers. (C) TEM images of chondrocytes showing mitochondrial ultrastructure in each group, with high-magnification views (bottom panels) indicating cristae integrity and membrane morphology. Data are presented as mean ± SD (n = 3); ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗P < 0.0001.

    Article Snippet: Rat chondrocytes (P1) were seeded in 24-well plates (5 × 10 4 cells per well) and cultured to ∼70–80% confluence, stimulated with LPS (10 μg mL −1 , 12 h) to induce an inflammatory phenotype, and then treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were washed three times with PBS, fixed in 4% paraformaldehyde for 15 min, permeabilized with 0.1% Triton X-100 (Aladdin, T434386) for 10 min, and blocked with 5% BSA (Solarbio, SW3015) at room temperature for 1 h. Primary antibodies were applied overnight at 4 °C: COL2A1 (Proteintech, 28459-1-AP, 1:200), SOX9 (HUABIO, HA723548, 1:1000), MMP9 (HUABIO, ET1704-69, 1:200), and ADAMTS5 (HUABIO, HA722011, 1:100).

    Techniques: In Vivo, Biomarker Discovery, Immunohistochemical staining, Staining, Expressing, Membrane