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antibodies against collagen i  (Proteintech)


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    Structured Review

    Proteintech antibodies against collagen i
    AAV‐mediated delivery of circIdh2 or circIdh2‐aa attenuated myocardial fibrosis and EndMT‐related marker changes in vivo. (A) HE staining of myocardial infarction tissues from the sham, model, circIdh2‐AAV, and circIdh2‐aa‐AAV groups. (B) Masson staining of MI tissues. (C) IF detection and quantification of fibrotic markers (Collagen I and TGF‐ β ) in myocardial tissues. Scale bars, 100 μ m. (D) Western blot analysis of EndMT‐related proteins (VE‐cadherin, vimentin, and N‐cadherin). Data are mean ± SD; n = 3 rats per group; one‐way ANOVA; ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
    Antibodies Against Collagen I, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 795 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+collagen+1/Collagen+Type+I+Antibody/pmc13647212-185-10-16
    Average 96 stars, based on 795 article reviews
    antibodies against collagen i - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "CircIdh2 Attenuates Endothelial‐to‐Mesenchymal Transition and Myocardial Fibrosis and Exhibits Translational Potential"

    Article Title: CircIdh2 Attenuates Endothelial‐to‐Mesenchymal Transition and Myocardial Fibrosis and Exhibits Translational Potential

    Journal: Cardiovascular Therapeutics

    doi: 10.1155/cdr/2005876

    AAV‐mediated delivery of circIdh2 or circIdh2‐aa attenuated myocardial fibrosis and EndMT‐related marker changes in vivo. (A) HE staining of myocardial infarction tissues from the sham, model, circIdh2‐AAV, and circIdh2‐aa‐AAV groups. (B) Masson staining of MI tissues. (C) IF detection and quantification of fibrotic markers (Collagen I and TGF‐ β ) in myocardial tissues. Scale bars, 100 μ m. (D) Western blot analysis of EndMT‐related proteins (VE‐cadherin, vimentin, and N‐cadherin). Data are mean ± SD; n = 3 rats per group; one‐way ANOVA; ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.
    Figure Legend Snippet: AAV‐mediated delivery of circIdh2 or circIdh2‐aa attenuated myocardial fibrosis and EndMT‐related marker changes in vivo. (A) HE staining of myocardial infarction tissues from the sham, model, circIdh2‐AAV, and circIdh2‐aa‐AAV groups. (B) Masson staining of MI tissues. (C) IF detection and quantification of fibrotic markers (Collagen I and TGF‐ β ) in myocardial tissues. Scale bars, 100 μ m. (D) Western blot analysis of EndMT‐related proteins (VE‐cadherin, vimentin, and N‐cadherin). Data are mean ± SD; n = 3 rats per group; one‐way ANOVA; ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Techniques Used: Marker, In Vivo, H&E Stain, Staining, Western Blot, Analysis

    Related Articles

    Immunohistochemical staining:

    Article Title: Ergosterol from edible fungi: Enhancing fatty acid oxidation via CPT1A to protect against diabetic kidney disease.
    Article Snippet: Background: Diabetic kidney disease (DKD), a common microvascular complication of diabetes mellitus, is recognized as a leading cause of end-stage renal disease.. Ergosterol, a natural sterol abundant in edible fungi, has shown pharmacological effects that may benefit DKD treatment.. However, its precise mechanisms of action remain elusive.

    Staining:

    Article Title: Ergosterol from edible fungi: Enhancing fatty acid oxidation via CPT1A to protect against diabetic kidney disease.
    Article Snippet: Background: Diabetic kidney disease (DKD), a common microvascular complication of diabetes mellitus, is recognized as a leading cause of end-stage renal disease.. Ergosterol, a natural sterol abundant in edible fungi, has shown pharmacological effects that may benefit DKD treatment.. However, its precise mechanisms of action remain elusive.

    Article Title: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling
    Article Snippet: .. For IHC and IF staining, paraffin sections of left lung tissue samples were incubated overnight at 4 °C with primary antibodies: anti-SNX3 (Servicebio, China, diluted 1:200, #K009182P), anti-α-SMA (Proteintech, China, diluted 1:200, #23081-1-AP), anti-SP-C (Proteintech, China, diluted 1:200, #10774-1-AP), anti-CD31 (Servicebio, China, diluted 1:200, #GB15063), anti-CD68 (Servicebio, China, diluted 1:200, #GB11309), anti-collagen 1 (Proteintech, China, diluted 1:200, #66761-1-Ig), anti-Wls (Proteintech, China, diluted 1:200, #23081-1-AP), anti-CK-1α (Santa Cruz, USA, diluted 1:200, #SC-74582), and anti-β-catenin (Proteintech, China, diluted 1:200, #51667-2-AP). .. After incubation, the samples were exposed to anti-CY3-tyramide (Servicebio, China, diluted 1:500, #G1223), anti-FITC-tyramide (Servicebio, China, diluted 1:500, #G1222), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Servicebio, China, #G1012).

    Incubation:

    Article Title: Ergosterol from edible fungi: Enhancing fatty acid oxidation via CPT1A to protect against diabetic kidney disease.
    Article Snippet: Background: Diabetic kidney disease (DKD), a common microvascular complication of diabetes mellitus, is recognized as a leading cause of end-stage renal disease.. Ergosterol, a natural sterol abundant in edible fungi, has shown pharmacological effects that may benefit DKD treatment.. However, its precise mechanisms of action remain elusive.

    Article Title: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling
    Article Snippet: .. For IHC and IF staining, paraffin sections of left lung tissue samples were incubated overnight at 4 °C with primary antibodies: anti-SNX3 (Servicebio, China, diluted 1:200, #K009182P), anti-α-SMA (Proteintech, China, diluted 1:200, #23081-1-AP), anti-SP-C (Proteintech, China, diluted 1:200, #10774-1-AP), anti-CD31 (Servicebio, China, diluted 1:200, #GB15063), anti-CD68 (Servicebio, China, diluted 1:200, #GB11309), anti-collagen 1 (Proteintech, China, diluted 1:200, #66761-1-Ig), anti-Wls (Proteintech, China, diluted 1:200, #23081-1-AP), anti-CK-1α (Santa Cruz, USA, diluted 1:200, #SC-74582), and anti-β-catenin (Proteintech, China, diluted 1:200, #51667-2-AP). .. After incubation, the samples were exposed to anti-CY3-tyramide (Servicebio, China, diluted 1:500, #G1223), anti-FITC-tyramide (Servicebio, China, diluted 1:500, #G1222), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Servicebio, China, #G1012).

    Article Title: Dexamethasone: a cheap and convenient way to alleviate subsequent stenosis after thermal injury on ureter
    Article Snippet: .. The sections were successively deparaffinized and hydrated, followed by antigen retrieval at 95 °C for 15 min and immersion in 10% hydrogen peroxide for 10 min. Next, the sections were blocked with 10% normal goat serum at room temperature for 30 min and incubated with primary antibodies using anti-IL-1β (1:500, AF5103; Affinity), anti-α-SMA (1:1,000; ab124964; Abcam), anti-collagen 1 (1:300; 14695-1-AP; Proteintech), anti-fibronectin (1:200; 66042-1-Ig; Proteintech) overnight at 4 °C. .. The sections underwent three rounds of washing with PBS for 5 minutes each, followed by a 50-minute incubation at room temperature with the biotinylated secondary antibody (OriGene, USA).

    other:

    Article Title: Plasma apolipoprotein E protein attenuates pulmonary fibrosis through LRP1 and PLAU dual receptor-mediated TGF-β/Smad inhibition.
    Article Snippet: Cell and lung tissue lysates were prepared using RIPA lysis buffer (Beyotime, Shanghai, China).

    Immunohistochemistry:

    Article Title: Targeting sorting nexin 3 to treat pulmonary fibrosis by dual modulating Wnt/β-catenin signaling
    Article Snippet: .. For IHC and IF staining, paraffin sections of left lung tissue samples were incubated overnight at 4 °C with primary antibodies: anti-SNX3 (Servicebio, China, diluted 1:200, #K009182P), anti-α-SMA (Proteintech, China, diluted 1:200, #23081-1-AP), anti-SP-C (Proteintech, China, diluted 1:200, #10774-1-AP), anti-CD31 (Servicebio, China, diluted 1:200, #GB15063), anti-CD68 (Servicebio, China, diluted 1:200, #GB11309), anti-collagen 1 (Proteintech, China, diluted 1:200, #66761-1-Ig), anti-Wls (Proteintech, China, diluted 1:200, #23081-1-AP), anti-CK-1α (Santa Cruz, USA, diluted 1:200, #SC-74582), and anti-β-catenin (Proteintech, China, diluted 1:200, #51667-2-AP). .. After incubation, the samples were exposed to anti-CY3-tyramide (Servicebio, China, diluted 1:500, #G1223), anti-FITC-tyramide (Servicebio, China, diluted 1:500, #G1222), and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Servicebio, China, #G1012).

    Fluorescence:

    Article Title: Hyperuricemia suppresses lumican, exacerbating adverse remodeling after myocardial infarction by promoting fibroblast phenotype transition.
    Article Snippet: .. The following antibodies were used: anti-Lumican (A11593, ABclonal, 1:200, Wuhan, China), anti-α-SMA (A5228, Sigma‒Aldrich, 1:200, St. Louis, MO), anti-collagen 1 (14695-1-AP, Proteintech, Wuhan, China), antifibronectin (A12932, ABclonal, 1:200), anti-CD68 (ab955, Abcam), anti-Ly6G (127602, Biolegend, San Diego, CA), anti-CD31 (561810, BD Biosciences, San Jose, CA), antic-TnT (A11826, Invitrogen, Carlsbad, CA), 488 nm Alexa fluorescence-conjugated secondary antibodies (A-11001, Thermo Fisher Scientific, Waltham, MA), and 555 nm Alexa fluorescence-conjugated secondary antibodies (A-21428, Thermo Fisher Scientific). .. According to the manufacturer’s instructions, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using an In Situ Apoptosis Detection Kit (11684817910, Roche, Basel, Switzerland).

    Article Title: Hyperuricemia suppresses lumican, exacerbating adverse remodeling after myocardial infarction by promoting fibroblast phenotype transition
    Article Snippet: .. The following antibodies were used: anti-Lumican (A11593, ABclonal, 1:200, Wuhan, China), anti-α-SMA (A5228, Sigma‒Aldrich, 1:200, St. Louis, MO), anti-collagen 1 (14695-1-AP, Proteintech, Wuhan, China), anti-fibronectin (A12932, ABclonal, 1:200), anti-CD68 (ab955, Abcam), anti-Ly6G (127602, Biolegend, San Diego, CA), anti-CD31 (561810, BD Biosciences, San Jose, CA), anti-c-TnT (A11826, Invitrogen, Carlsbad, CA), 488 nm Alexa fluorescence-conjugated secondary antibodies (A-11001, Thermo Fisher Scientific, Waltham, MA), and 555 nm Alexa fluorescence-conjugated secondary antibodies (A-21428, Thermo Fisher Scientific). .. According to the manufacturer’s instructions, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was performed using an In Situ Apoptosis Detection Kit (11684817910, Roche, Basel, Switzerland).



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    Image Search Results


    AAV‐mediated delivery of circIdh2 or circIdh2‐aa attenuated myocardial fibrosis and EndMT‐related marker changes in vivo. (A) HE staining of myocardial infarction tissues from the sham, model, circIdh2‐AAV, and circIdh2‐aa‐AAV groups. (B) Masson staining of MI tissues. (C) IF detection and quantification of fibrotic markers (Collagen I and TGF‐ β ) in myocardial tissues. Scale bars, 100 μ m. (D) Western blot analysis of EndMT‐related proteins (VE‐cadherin, vimentin, and N‐cadherin). Data are mean ± SD; n = 3 rats per group; one‐way ANOVA; ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Journal: Cardiovascular Therapeutics

    Article Title: CircIdh2 Attenuates Endothelial‐to‐Mesenchymal Transition and Myocardial Fibrosis and Exhibits Translational Potential

    doi: 10.1155/cdr/2005876

    Figure Lengend Snippet: AAV‐mediated delivery of circIdh2 or circIdh2‐aa attenuated myocardial fibrosis and EndMT‐related marker changes in vivo. (A) HE staining of myocardial infarction tissues from the sham, model, circIdh2‐AAV, and circIdh2‐aa‐AAV groups. (B) Masson staining of MI tissues. (C) IF detection and quantification of fibrotic markers (Collagen I and TGF‐ β ) in myocardial tissues. Scale bars, 100 μ m. (D) Western blot analysis of EndMT‐related proteins (VE‐cadherin, vimentin, and N‐cadherin). Data are mean ± SD; n = 3 rats per group; one‐way ANOVA; ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Article Snippet: The sections were then incubated overnight at 4°C with primary antibodies against Collagen I (1:200, #66761‐1‐Ig, Proteintech) and TGF‐ β 1 (1:200, #81746‐2‐RR, Proteintech).

    Techniques: Marker, In Vivo, H&E Stain, Staining, Western Blot, Analysis

    qPCR primer information.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: pH-responsive Zn 2+ -coordinated gellan gum/γ-polyglutamic acid hydrogel microspheres as functional biomaterials for intra-articular delivery: physicochemical design, smart release, and chondroprotective performance in osteoarthritis

    doi: 10.3389/fbioe.2026.1924086

    Figure Lengend Snippet: qPCR primer information.

    Article Snippet: The sections were subsequently reacted with primary antibodies targeting COL2A1 (Proteintech, 28459-1-AP), MMP13 (Proteintech, 18165-1-AP), p-PI3K (HUABIO, HA721672), and p-AKT (Proteintech, 66444-1-Ig) for 16 h at 4 °C.

    Techniques: Sequencing

    PGG-Zn@Cur restored matrix metabolic homeostasis in IL-1β-stimulated chondrocytes. (A) Representative immunofluorescence images of COL2A1 in chondrocytes after different treatments. COL2A1 was stained red, and nuclei were counterstained with DAPI. (B) Representative immunofluorescence images of MMP13 in chondrocytes after different treatments. MMP13 was stained green, and nuclei were counterstained with DAPI. (C) Quantitative analysis of relative fluorescence intensity for COL2A1 and MMP13 based on immunofluorescence staining. IL-1β stimulation decreased COL2A1 expression and increased MMP13 accumulation, whereas PGG-Zn@Cur treatment restored COL2A1 fluorescence intensity and suppressed MMP13-positive signals. (D–G) RT-qPCR analysis of cartilage matrix metabolism-related genes, including anabolic markers Col2a1 and Aggrecan, and catabolic markers Mmp13 and Adamts5, in chondrocytes after the indicated treatments. (H) Representative Western blot images of iNOS, COL2A1, MMP13, and ACAN protein expression, together with heatmap-based quantitative analysis of Western blot results across different treatment groups. β-Actin was used as the loading control. ( n = 3, Scale bar: 10 μm *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: no significant).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: pH-responsive Zn 2+ -coordinated gellan gum/γ-polyglutamic acid hydrogel microspheres as functional biomaterials for intra-articular delivery: physicochemical design, smart release, and chondroprotective performance in osteoarthritis

    doi: 10.3389/fbioe.2026.1924086

    Figure Lengend Snippet: PGG-Zn@Cur restored matrix metabolic homeostasis in IL-1β-stimulated chondrocytes. (A) Representative immunofluorescence images of COL2A1 in chondrocytes after different treatments. COL2A1 was stained red, and nuclei were counterstained with DAPI. (B) Representative immunofluorescence images of MMP13 in chondrocytes after different treatments. MMP13 was stained green, and nuclei were counterstained with DAPI. (C) Quantitative analysis of relative fluorescence intensity for COL2A1 and MMP13 based on immunofluorescence staining. IL-1β stimulation decreased COL2A1 expression and increased MMP13 accumulation, whereas PGG-Zn@Cur treatment restored COL2A1 fluorescence intensity and suppressed MMP13-positive signals. (D–G) RT-qPCR analysis of cartilage matrix metabolism-related genes, including anabolic markers Col2a1 and Aggrecan, and catabolic markers Mmp13 and Adamts5, in chondrocytes after the indicated treatments. (H) Representative Western blot images of iNOS, COL2A1, MMP13, and ACAN protein expression, together with heatmap-based quantitative analysis of Western blot results across different treatment groups. β-Actin was used as the loading control. ( n = 3, Scale bar: 10 μm *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: no significant).

    Article Snippet: The sections were subsequently reacted with primary antibodies targeting COL2A1 (Proteintech, 28459-1-AP), MMP13 (Proteintech, 18165-1-AP), p-PI3K (HUABIO, HA721672), and p-AKT (Proteintech, 66444-1-Ig) for 16 h at 4 °C.

    Techniques: Immunofluorescence, Staining, Analysis, Fluorescence, Expressing, Quantitative RT-PCR, Western Blot, Control

    PGG-Zn@Cur attenuated PI3K-AKT activation and apoptosis-associated responses in IL-1β-stimulated chondrocytes. (A) Schematic workflow of RNA sequencing and subsequent experimental validation. (B) Heatmap of representative differentially expressed genes related to cartilage matrix metabolism, inflammation, apoptosis, and PI3K-AKT-associated signaling. (C) Volcano plot showing differentially expressed genes between the indicated groups, with representative genes related to cartilage homeostasis, apoptosis, and PI3K-AKT signaling labeled. (D) GO enrichment analysis of differentially expressed genes, including biological process, cellular component, and molecular function categories. (E) KEGG pathway enrichment analysis, with the PI3K-AKT signaling pathway highlighted as a candidate pathway. (F) Representative immunofluorescence images of p-PI3K, p-AKT, Cl-Caspase3, and COL2A1 in chondrocytes after different treatments. Nuclei were counterstained with DAPI. (G) Quantitative analysis of p-PI3K, p-AKT, Cl-Caspase3, and COL2A1 fluorescence intensity. (H) Representative Western blot images of PI3K-AKT pathway- and apoptosis-related proteins, including p-PI3K, PI3K, p-AKT, AKT, Cleaved-Caspase3, Caspase3, Bax, and BCL2. β-Actin was used as the loading control. (I) Heatmap-based quantitative analysis of Western blot results, summarizing changes in p-PI3K/PI3K, p-AKT/AKT, Cleaved-Caspase3/Caspase3, Bax/β-Actin, and BCL2/β-Actin across different groups. ( n = 3, Scale bar: 10 μm *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: not significant).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: pH-responsive Zn 2+ -coordinated gellan gum/γ-polyglutamic acid hydrogel microspheres as functional biomaterials for intra-articular delivery: physicochemical design, smart release, and chondroprotective performance in osteoarthritis

    doi: 10.3389/fbioe.2026.1924086

    Figure Lengend Snippet: PGG-Zn@Cur attenuated PI3K-AKT activation and apoptosis-associated responses in IL-1β-stimulated chondrocytes. (A) Schematic workflow of RNA sequencing and subsequent experimental validation. (B) Heatmap of representative differentially expressed genes related to cartilage matrix metabolism, inflammation, apoptosis, and PI3K-AKT-associated signaling. (C) Volcano plot showing differentially expressed genes between the indicated groups, with representative genes related to cartilage homeostasis, apoptosis, and PI3K-AKT signaling labeled. (D) GO enrichment analysis of differentially expressed genes, including biological process, cellular component, and molecular function categories. (E) KEGG pathway enrichment analysis, with the PI3K-AKT signaling pathway highlighted as a candidate pathway. (F) Representative immunofluorescence images of p-PI3K, p-AKT, Cl-Caspase3, and COL2A1 in chondrocytes after different treatments. Nuclei were counterstained with DAPI. (G) Quantitative analysis of p-PI3K, p-AKT, Cl-Caspase3, and COL2A1 fluorescence intensity. (H) Representative Western blot images of PI3K-AKT pathway- and apoptosis-related proteins, including p-PI3K, PI3K, p-AKT, AKT, Cleaved-Caspase3, Caspase3, Bax, and BCL2. β-Actin was used as the loading control. (I) Heatmap-based quantitative analysis of Western blot results, summarizing changes in p-PI3K/PI3K, p-AKT/AKT, Cleaved-Caspase3/Caspase3, Bax/β-Actin, and BCL2/β-Actin across different groups. ( n = 3, Scale bar: 10 μm *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: not significant).

    Article Snippet: The sections were subsequently reacted with primary antibodies targeting COL2A1 (Proteintech, 28459-1-AP), MMP13 (Proteintech, 18165-1-AP), p-PI3K (HUABIO, HA721672), and p-AKT (Proteintech, 66444-1-Ig) for 16 h at 4 °C.

    Techniques: Activation Assay, RNA Sequencing, Biomarker Discovery, Labeling, Analysis, Immunofluorescence, Fluorescence, Western Blot, Control

    PGG-Zn@Cur attenuates OA-associated cartilage degeneration and preserves matrix homeostasis in vivo . (A) Representative Safranin O/Fast Green staining images of knee joint cartilage from the Sham, OA, OA+ PGG-Zn, OA+ Cur, and OA+ PGG-Zn@Cur groups. (B) Representative immunofluorescence images of COL2A1 and MMP13 in articular cartilage from different groups. Nuclei were counterstained with DAPI. (C, D) Quantitative analysis of cartilage degeneration using OARSI scores and modified Mankin’s scores. (E) Quantitative analysis of COL2A1 and MMP13 fluorescence intensity in articular cartilage. ( n = 5 mice per group, Scale bar: 50 μm *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: not significant).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: pH-responsive Zn 2+ -coordinated gellan gum/γ-polyglutamic acid hydrogel microspheres as functional biomaterials for intra-articular delivery: physicochemical design, smart release, and chondroprotective performance in osteoarthritis

    doi: 10.3389/fbioe.2026.1924086

    Figure Lengend Snippet: PGG-Zn@Cur attenuates OA-associated cartilage degeneration and preserves matrix homeostasis in vivo . (A) Representative Safranin O/Fast Green staining images of knee joint cartilage from the Sham, OA, OA+ PGG-Zn, OA+ Cur, and OA+ PGG-Zn@Cur groups. (B) Representative immunofluorescence images of COL2A1 and MMP13 in articular cartilage from different groups. Nuclei were counterstained with DAPI. (C, D) Quantitative analysis of cartilage degeneration using OARSI scores and modified Mankin’s scores. (E) Quantitative analysis of COL2A1 and MMP13 fluorescence intensity in articular cartilage. ( n = 5 mice per group, Scale bar: 50 μm *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: not significant).

    Article Snippet: The sections were subsequently reacted with primary antibodies targeting COL2A1 (Proteintech, 28459-1-AP), MMP13 (Proteintech, 18165-1-AP), p-PI3K (HUABIO, HA721672), and p-AKT (Proteintech, 66444-1-Ig) for 16 h at 4 °C.

    Techniques: In Vivo, Staining, Immunofluorescence, Analysis, Modification, Fluorescence

    PGG-Zn@Cur attenuates PI3K-AKT pathway activation and apoptosis-associated cartilage damage in vivo . (A) Representative immunofluorescence images of p-PI3K, p-AKT, and COL2A1 in articular cartilage from the Sham, OA, OA+ PGG-Zn, OA+ Cur, and OA+ PGG-Zn@Cur groups. Nuclei were counterstained with DAPI. (B) Representative TUNEL staining images of articular cartilage from different groups. (C) Quantitative analysis of p-PI3K, p-AKT, and COL2A1 fluorescence intensity in articular cartilage. (D) Quantitative analysis of TUNEL-positive chondrocytes in articular cartilage. ( n = 5 mice per group, Scale bar: 50 μm *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: not significant).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: pH-responsive Zn 2+ -coordinated gellan gum/γ-polyglutamic acid hydrogel microspheres as functional biomaterials for intra-articular delivery: physicochemical design, smart release, and chondroprotective performance in osteoarthritis

    doi: 10.3389/fbioe.2026.1924086

    Figure Lengend Snippet: PGG-Zn@Cur attenuates PI3K-AKT pathway activation and apoptosis-associated cartilage damage in vivo . (A) Representative immunofluorescence images of p-PI3K, p-AKT, and COL2A1 in articular cartilage from the Sham, OA, OA+ PGG-Zn, OA+ Cur, and OA+ PGG-Zn@Cur groups. Nuclei were counterstained with DAPI. (B) Representative TUNEL staining images of articular cartilage from different groups. (C) Quantitative analysis of p-PI3K, p-AKT, and COL2A1 fluorescence intensity in articular cartilage. (D) Quantitative analysis of TUNEL-positive chondrocytes in articular cartilage. ( n = 5 mice per group, Scale bar: 50 μm *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001; ns: not significant).

    Article Snippet: The sections were subsequently reacted with primary antibodies targeting COL2A1 (Proteintech, 28459-1-AP), MMP13 (Proteintech, 18165-1-AP), p-PI3K (HUABIO, HA721672), and p-AKT (Proteintech, 66444-1-Ig) for 16 h at 4 °C.

    Techniques: Activation Assay, In Vivo, Immunofluorescence, TUNEL Assay, Staining, Analysis, Fluorescence